1352510462530 7fffb663120dd173020ed4b9bc223583

Donald Danforth Plant Science Center

St. Louis, MO, US
1352510432255 7fffb663120dd173020ed4b9bc223583

Proteomics and Mass Spectrometry Facility

St. Louis Missouri
107 Orders Completed
97% Positive

The Danforth Center’s Proteomics & Mass Spectrometry Facility develops new tools and provides high quality sample analysis in proteomics, mass spectrometry and related analytical fields.

The Proteomics & Mass Spectrometry Facility, under the direction of Dr. Bradley... Show more »

The Danforth Center’s Proteomics & Mass Spectrometry Facility develops new tools and provides high quality sample analysis in proteomics, mass spectrometry and related analytical fields.

The Proteomics & Mass Spectrometry Facility, under the direction of Dr. Bradley Evans, has processed thousands of service samples submitted from research laboratories located at the Danforth Center as well as from academic and commercial institutions worldwide. In addition, the facility has initiated research activities designed to expand analytical capabilities and has established collaborations with many principal investigators at various institutions. These activities have produced exciting results and publications, which are a testament to the facility's high standards and growing reputation.

With highly specialized capabilities and research initiatives, the facility is well-prepared and positioned to serve as a regional, national and international resource in the fast developing fields of proteomics and metabolomics.

We have an in-house collection of microorganisms too numerous to list; as a plant-science focused institution we of course also have access to plants, including, but not limited to: Arabidopsis, Setaria viridis, sorghum, soy bean, tomato, corn, Camelina, Cassava, others. We can and have also worked with human and animal primary tissue and cell-lines.

Instrumentation:
- LTQ-Orbitrap Velos Pro with 3000 RSLCnano-LC or Advion Triversa,
-6500 QTrap with Shimadzu UFLC-XR UHPLC and Eksigent 200 MicroLC
-Q-Exactive with Agilent 1200 CapLC and Eksigent NanoLC 2D Ultra
-Agilent 5975 GC-MS
-Thermo ITQ 900 GC-MS/FID
-Thermo Trace GC-FID
-Waters ACQUITY UPLC with Acutag for amino acid analysis
-Beckman System-GOLD, Agilent 1100 and Shimadzu Prominence HPLC systems for offline fractionation or UV-Vis/fluorescence analysis
-Akta Explorer FPLC

Publications:
http://www.danforthcenter.org/scientists-research/core-technologies/proteomics-mass-spectrometry/publications

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Integrated Microscopy Facility

St. Louis MO
No Reviews Available

The Donald Danforth Plant Science Center's Integrated Microscopy Facility furnishes up-to-date instrumentation for cellular imaging - we encourage you to contact us for your imaging needs.

The facility’s premiere instrument for live cell imaging is a Leica SP8-X confocal... Show more »

The Donald Danforth Plant Science Center's Integrated Microscopy Facility furnishes up-to-date instrumentation for cellular imaging - we encourage you to contact us for your imaging needs.

The facility’s premiere instrument for live cell imaging is a Leica SP8-X confocal microscope equipped with white light laser, resonance scanner and HyD detectors. In addition to this we have other fluorescence microscopes to meet our users' needs.

The facility has a workstation for confocal image processing, equipped with Bitplane Imaris (for 3D and 4D image reconstruction, tracking, and colocalization analysis) and SVI’s Huygens deconvolution software (for improved signal:noise and resolution). The Nyquist calculator can be used to determine the correct settings on the confocal microscope to meet Nyquist resolution standards.

Confocal microscopy requires a solid background in understanding how to optimally acquire image data and prepare it for publication. To that end, the facility offers comprehensive five day confocal microscopy workshops in January and July.

The facility's electron microscopes include a LEO 912 AB energy-filter transmission electron microscope (TEM) and a Hitachi TM-1000 tabletop SEM. We have state-of-the-art specimen preparation for TEM: ultra-rapid freezing done using a Bal-Tec high pressure freezer. We also have the ancillary equipment required for transmission electron microscopy.

A prep lab and darkroom are included in the suite, as are computers for digital processing of images using Imaris software.

The facility director R.H. Berg has considerable experience in the study of plant cell structure and he serves as a resource for users in designing and implementing their imaging experiments.

Services are provided to any interested party and are designed with convenience in mind for the client. For those wanting to use the instrument themselves, qualifying for the self-service fee schedule, this can be done after training by IMF staff. On the other hand, full service is also available, putting the work in the hand of our experienced staff.

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