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Histopathology & Tissue Shared Resource (HTSR)

Washington District of Columbia

Located at Georgetown University Medical Center, we provide comprehensive histology, pathology and imaging services. Our Human Tissue Bank supports translational research through the provision of patient tissue samples from archival paraffin blocks or fresh frozen tissue samples. Our histology services includes tissue fixation/processing, embedding, microtomy and staining services for both frozen and paraffin embedded tissues. We support colorimetric and fluorescent IHC staining for established antibodies as well as for the development of protocols for new antibodies. The IHC lab also supports TUNEL labeling and novel labeling techniques. High throughput tissue processors and autostainers for both H&E and IHC facilitate the production of highly precise and accurate tissue samples and stains combined with rapid turnaround.

Histopathology & Tissue Shared Resource (HTSR) has not listed any services.

Immunohistochemistry (IHC)
Starting at $19.00 per slide

Immunological staining of tissue sections. Development of novel staining procedures.
Services:
- 2 DAKO Autostainers for high throughput precise immunohistochemical staining.
- Protocol development using new antibodies from commercial vendors or custom antibodies.
- Optimization of staining intensity and... Show more »

Immunological staining of tissue sections. Development of novel staining procedures.
Services:
- 2 DAKO Autostainers for high throughput precise immunohistochemical staining.
- Protocol development using new antibodies from commercial vendors or custom antibodies.
- Optimization of staining intensity and specificity.

Deliverables:
- Publication quality immuno-stained slides
- Optimized staining protocols for established and novel antibodies

Turn around time:
For Established antibodies - 1-2 weeks
For Novel antibodies - 1 month

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Agilent DAKO
H&E Staining
Hematoxylin and eosin staining
Starting at $6.50 per slide

Includes sectioning and H&E staining of paraffin embedded sample

Related services available - Sectioning and H&E Staining of frozen tissue blocks ($8.00 USD per slide)

Deliverables:
- stained slides

Turn around time - 1-2 weeks

Includes sectioning and H&E staining of paraffin embedded sample

Related services available - Sectioning and H&E Staining of frozen tissue blocks ($8.00 USD per slide)

Deliverables:
- stained slides

Turn around time - 1-2 weeks

« Show less
Special slide staining
Starting at $11.00 per slide

Staining of slides using dye based histopathology. Stains include but are not limited to:
Masson's Trichrome
Mast Cell Staining (Chloroacetate esterase)
Periodic Acid Schiff's (PAS)
Toluidine Blue
Nissl Stain
Gram Stain
Sirius Red

Deliverables:
- stained slides

Turn around time - 1-2 weeks

Staining of slides using dye based histopathology. Stains include but are not limited to:
Masson's Trichrome
Mast Cell Staining (Chloroacetate esterase)
Periodic Acid Schiff's (PAS)
Toluidine Blue
Nissl Stain
Gram Stain
Sirius Red

Deliverables:
- stained slides

Turn around time - 1-2 weeks

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Immunofluorescence
Starting at $25.00 per slide

Optimization of multi-label fluorescent based immunostains. Up to 4 different fluorescent labels available (DAPI, GFP/488, Cy3/555, Cy5)
Equipment: Confocal
Objective lenses - 4X, 10X, 20X, 40X, 60X oil, 100X oil
Fluorescent channels and wavelengths - DAPI, GFP, Cy3, Cy5

Turn around time for full optimization of... Show more »

Optimization of multi-label fluorescent based immunostains. Up to 4 different fluorescent labels available (DAPI, GFP/488, Cy3/555, Cy5)
Equipment: Confocal
Objective lenses - 4X, 10X, 20X, 40X, 60X oil, 100X oil
Fluorescent channels and wavelengths - DAPI, GFP, Cy3, Cy5

Turn around time for full optimization of multi-labeled stain - 1 month

We do not:
- culture mammalian cells or prepare them for antibody staining
- culture non-mammalian (i.e. insect) cells
- transfect cells with either viruses or chemicals
- image live cells

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TUNEL Assay
Terminal deoxynucleotidyl transferase dUTP nick end labeling assay
Starting at $32.00 per sample

TUNEL is used to detect apoptotic cells in tissue sections.

Deliverables - TUNEL labeled slides along with known positive and negative control samples.

Turn around time - 1-2 weeks

TUNEL is used to detect apoptotic cells in tissue sections.

Deliverables - TUNEL labeled slides along with known positive and negative control samples.

Turn around time - 1-2 weeks

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Paraffin Embedding
Starting at $7.50 per sample

Processing and sectioning. Routine and non-routine histology stains.

Related services - Sectioning for unstained slides ($5.25 US) and H&E stained ($6.50 US) sides

Deliverables - Paraffin embedded tissue blocks

Turn around time - 1 week

Processing and sectioning. Routine and non-routine histology stains.

Related services - Sectioning for unstained slides ($5.25 US) and H&E stained ($6.50 US) sides

Deliverables - Paraffin embedded tissue blocks

Turn around time - 1 week

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Paraffin Sectioning
Starting at $5.25 per slide

Sectioning of paraffin embedded tissue to produce unstained slides.
Unstained slides can be used for IHC or dye based staining

Related services - Cyrosectioning of frozen samples

Turn around time - 1-2 weeks

Sectioning of paraffin embedded tissue to produce unstained slides.
Unstained slides can be used for IHC or dye based staining

Related services - Cyrosectioning of frozen samples

Turn around time - 1-2 weeks

« Show less
Cryoembedding
Starting at $11.25 per sample

Embedding of fresh or fixed samples into OCT embedded blocks.

Deliverables - Tissue sample frozen in OCT block

Related services available - Cryosectioning for H&E and unstained slides

Turn around time - 1 week

Embedding of fresh or fixed samples into OCT embedded blocks.

Deliverables - Tissue sample frozen in OCT block

Related services available - Cryosectioning for H&E and unstained slides

Turn around time - 1 week

« Show less
Cryosectioning
Starting at $6.50 per slide

Sectioning of unstained frozen tissue samples.

Deliverables - Unstained frozen tissue section ready for IHC or dye based staining

Related services available - Cyro-embedding and Cryosectioning of frozen sample followed by H&E staining ($8.00 per slide)

Turn around time - 1-2 weeks

Sectioning of unstained frozen tissue samples.

Deliverables - Unstained frozen tissue section ready for IHC or dye based staining

Related services available - Cyro-embedding and Cryosectioning of frozen sample followed by H&E staining ($8.00 per slide)

Turn around time - 1-2 weeks

« Show less
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July 05, 2017

Net Promoter Score of 10 received for Paraffin Sectioning.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 8.
July 05, 2017

Net Promoter Score of 10 received for Special slide staining.

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 8.
December 20, 2016

Net Promoter Score of 10 received for Immunohistochemistry (IHC).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 9.
September 29, 2016

Net Promoter Score of 9 received for Immunohistochemistry (IHC).

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 9.
July 11, 2016

Positive review received for H&E Staining:

"Deb was very helpful in solving problems with our mouse histology samples. We are very happy with their work quality. I would strongly recommend her lab to others."

June 21, 2016

Net Promoter Score of 8 received for Immunohistochemistry (IHC).

Additional Ratings: satisfaction with deliverable: 8, satisfaction with timeliness: 10.
June 21, 2016

Net Promoter Score of 9 received for Immunohistochemistry (IHC).

Additional Ratings: satisfaction with deliverable: 10, satisfaction with timeliness: 8.
June 20, 2016

Net Promoter Score of 9 received for Paraffin Sectioning.

Additional Ratings: satisfaction with deliverable: 9, satisfaction with timeliness: 10.
June 30, 2015

Positive review received for Immunohistochemistry (IHC):

"Excellent work, as always!"

June 19, 2015

Positive review received for Immunohistochemistry (IHC):

"Excellent work!"

January 14, 2015

Positive review received for Immunohistochemistry (IHC):

"We were very happy with the IHC protocol development and staining that was performed at Histopathology and Tissue Shared Resources. Deborah was great to work with."

March 19, 2014

Positive review received for Immunohistochemistry (IHC):

"Deb was great to work with! She was fast, responsive, and very communicative. She even did a bit of extra work above and beyond the requirements of the project, and the data she returned was very clean and clear. I'm looking forward to working with Deb on more projects!"

April 22, 2013

Positive review received for Immunohistochemistry (IHC):

"The staining looks great!"

March 27, 2013

Positive review received for Immunohistochemistry (IHC):

"Deborah provides excellent service!"

December 12, 2011

Positive review received for Histology & Pathology:

"Deborah was prompt and professional in all interactions and the quality of work was exceptional."

October 4, 2011

Positive review received for Histology & Pathology:

"Deborah was very reliable, professional, and communicative. Our lab was very happy with the quality, turn around time, and price for service. I certainly recommend Deborah and the services of the Lombardi Institute to anyone."

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